Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14684] to DISC1 - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DISC1 antibody [EPR14684] - BSA and Azide free
    See all DISC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14684] to DISC1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody has cross-reactivity with GST, so we suggest not to use this antibody for detecting protein with a GST-tag.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human brain, mouse brain and human adenocarcinoma of endometrium tissue. ICC/IF: Neuro-2a cells. Flow Cyt: Neuro-2a cells. IP: U87-MG cell lysate.

  • General notes

    Ab245775 is the carrier-free version of ab192258. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245775 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)
    Flow Cytometry - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)

    Flow cytometry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling DISC1 (red) with purified ab192258 at a 1/1000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab192258).

  • Immunoprecipitation - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)
    Immunoprecipitation - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)

    Lysate from U87-MG cells was immunoprecipitated with ab192258 at a 1/60 dilution. For the subsequent blot, ab192258 used at a 1/1000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab192258).

  • Immunocytochemistry/ Immunofluorescence - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)
    Immunocytochemistry/ Immunofluorescence - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)

    Immunofluorescent analysis of 4% formaldehyde fixed Neuro-2a cells labeling DISC1 using ab192258 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) ab150077 was used as the secondary at a 1/200 dilution. Permeabilized using 0.1% Triton X-100. Counterstained with DAPI.  The two negative controls: 1. Primary ab concentration (anti-DISC1 ) is 1:1000 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution; 2. Primary ab concentration (anti-DISC1 ) is 1:1000 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab192258).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)

    Immunohistochemical analysis of paraffin embedded human brain tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab192258).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)

    Immunohistochemical analysis of paraffin embedded Human adenocarcinoma of endometrium tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; glycerol and sodium azide (ab192258)

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)

    Immunohistochemical analysis of paraffin embedded mouse brain tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab192258).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)
    Anti-DISC1 antibody [EPR14684] - BSA and Azide free (ab245775)

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