Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12378] to PAR6 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PAR6 antibody [EPR12378] - BSA and Azide free
    See all PAR6 primary antibodies

  • Description

    Rabbit monoclonal [EPR12378] to PAR6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250164 is the carrier-free version of ab180159. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250164 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)
    Western blot - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)
    All lanes : Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : Y79 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 37 kDa



    This data was developed using ab180159, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)
    Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)

    This data was developed using ab180159, the same antibody clone in a different buffer formulation.

    Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing PAR6, using ab180159 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)
    Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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