Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in Hela Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

All lanes : Anti-AP2 gamma/TFAP2C antibody [3B5] (ab110635) at 1/50 dilution

Lane 1 : PC-3 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Mouse placenta cell lysate
Lane 4 : RAW 264.7 cell lysate

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : Goat anti-Mouse IgG (H+L), HRP conjugate at 1/4000 dilution

Predicted band size: 49 kDa

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with ab110635 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in U251 Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in MCF-7 Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.