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Cancer Cell cycle Cell differentiation

Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Price and availability

324 988 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2692Y] to AP2 gamma/TFAP2C
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-AP2 gamma/TFAP2C antibody [EP2692Y]
    See all AP2 gamma/TFAP2C primary antibodies
  • Description

    Rabbit monoclonal [EP2692Y] to AP2 gamma/TFAP2C
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human AP2 gamma/TFAP2C aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Purchase matching WB positive control:Recombinant Human AP2 gamma/TFAP2C protein
    • WB: MCF7 and SKBR3 cell lysates. IHC-P: Human cervical carcinoma, endometrial carcinoma and prostatic carcinoma tissues. ICC/IF: MCF7 cells Flow Cyt: MCF7 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as AP2 gamma

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP2692Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
    Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
    All lanes : Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007) at 1/50000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : SKBR3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
    Immunocytochemistry/ Immunofluorescence - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling AP2 gamma/TFAP2C with purified ab76007 at 1/500 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

    ab76007 showing positive staining in Cervical carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
    Flow Cytometry - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling AP2 gamma/TFAP2C with purified ab76007 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

    ab76007 showing positive staining in Endometrial carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

    ab76007 showing positive staining in Prostatic carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)
    Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: IHC-P

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