Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20331] to AP2 gamma/TFAP2C
Suitable for: ICC/IF, Flow Cyt, WB, IHC-P, IP
Reacts with: Human

Product name

Anti-AP2 gamma/TFAP2C antibody [EPR20331]
See all AP2 gamma/TFAP2C primary antibodies
Description

Rabbit monoclonal [EPR20331] to AP2 gamma/TFAP2C
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: SK-BR-3, MDA-MB-435S, MCF7 and T-47D whole cell lysates. IHC-P: Human breast, placenta, breast cancer and seminoma tissues. ICC/IF: SK-BR-3 and MCF7 cells. Flow Cyt: SK-BR-3 cells. IP: MCF7 whole cell lysate.
General notes
This product was previously labelled as AP2 gamma

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20331
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Nuclear staining on human normal breast is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-BR-3 (Human mammary gland adenocarcinoma cell line) cells labeling AP2 gamma/TFAP2C with ab218107 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on SK-BR-3 cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Western blot - Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

Lanes 1-2 : Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107) at 1/1000 dilution
Lanes 3-4 : Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107) at 1/2000 dilution

Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate
Lane 2 : MDA-MB-435S (Human ductal carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 49 kDa
Observed band size: 49 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/2: 30 seconds; Lane 3: 10 seconds; Lane 4: 3 minutes.
Flow Cytometry - Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

Flow cytometric analysis of 4% paraformaldehyde-fixed SK-BR-3 (Human mammary gland adenocarcinoma cell line) cells labeling AP2 gamma/TFAP2C with ab218107 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

AP2 gamma/TFAP2C was immunoprecipitated from 0.35 mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab218107 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab218107 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: MCF7 whole cell lysate, 10 µg (Input).

Lane 2: ab218107 IP in MCF7 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab2018107 in MCF7 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Nuclear staining on human placenta is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Nuclear staining on poorly differentiated human breast cancer is observed. The data is consistent with the literature (PMID: 18825690).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AP2 gamma/TFAP2C with ab218107 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on MCF7 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Nucleus staining on human seminoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Almost all tumor cells show negative staining on moderately differentiated human breast cancer. The data is consistent with the literature (PMID: 18825690).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107)

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