Anti-Axin 2 antibody (ab107613)
Key features and details
- Rabbit polyclonal to Axin 2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Axin 2 antibody
See all Axin 2 primary antibodies -
Description
Rabbit polyclonal to Axin 2 -
Host species
Rabbit -
Specificity
ab107613 antibody is predicted to not cross-react with Axin 1. At least four isoforms of Axin 2 are known to exist; this antibody will recognize all four. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Axin 2 (C terminal). The immunogen is located within amino acids 780 - 830 of Axin 2.
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Positive control
- Mouse lung tissue lysate. This antibody gave a positive result in IF/ICC when used in the following methanol fixed cell lines: PC12.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody (ab107613)
Immunohistochemical analysis of Axin 2 expression in rat lung cells with ab107613 at 20 µg/mL.
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Western blot - Anti-Axin 2 antibody (ab107613)Anti-Axin 2 antibody (ab107613) at 1 µg/ml + Mouse lung tissue lysate at 15 µg
Predicted band size: 94 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab107613)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse lung tissue staining Axin 2 with ab107613 at 20 µg/mL.
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Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody (ab107613)Immunohistochemical analysis of Axin 2 expression in HepG2 cells with ab107613 at 20 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab107613)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse lung tissue labeling Axin 2 with Anti-Axin 2 antibody (ab107613) at 5μg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab107613)Immunohistochemistry of AXIN2 in rat lung tissue with ab107613 at 5 µg/ml -
Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody (ab107613)ICC/IF image of ab107613 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107613 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab107613) This image is courtesy of an anonymous Abreviewab107613 staining Axin 2 in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/800) was used as the secondary antibody.
