Anti-Axin 2 antibody (ab32197)
Key features and details
- Rabbit polyclonal to Axin 2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Axin 2 antibody
See all Axin 2 primary antibodies -
Description
Rabbit polyclonal to Axin 2 -
Host species
Rabbit -
Specificity
Milk blocking not recommended -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Rat, Chicken
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Immunogen
Synthetic peptide corresponding to Mouse Axin 2 aa 800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab32196)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab32197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 0.1 - 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa) in endogenous lysates. Ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa. Target
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Function
Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B. -
Tissue specificity
Expressed in brain and lymphoblast. -
Involvement in disease
Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types. -
Sequence similarities
Contains 1 DIX domain.
Contains 1 RGS domain. -
Domain
The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2. -
Post-translational
modificationsProbably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8313 Human
- Entrez Gene: 12006 Mouse
- Entrez Gene: 29134 Rat
- Omim: 604025 Human
- SwissProt: Q9Y2T1 Human
- SwissProt: O88566 Mouse
- SwissProt: O70240 Rat
- Unigene: 156527 Human
see all -
Alternative names
- Axil antibody
- Axin like protein antibody
- Axin-2 antibody
see all
Images
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Western blot - Anti-Axin 2 antibody (ab32197)Anti-Axin 2 antibody (ab32197) at 1 µg/ml + AXIN2 partial recombinant tagged protein at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 94 kDaab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab32197)IHC image of ab32197 stained area of normal tissue in a human colorectal cancer FFPE section. Section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 seconds at 125°C. Section was incubated with ab32197 at a dilution of 1:500 for 1h at room temperature and detected using an HRP conjugated polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Western blot - Anti-Axin 2 antibody (ab32197)All lanes : Anti-Axin 2 antibody (ab32197) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : Colon (Mouse) Tissue Lysate
Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 4 : LS147T (Colon cancer cells) (Human) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 94 kDa
Observed band size: 94 kDa
Additional bands at: 32 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Protocols
References (55)
ab32197 has been referenced in 55 publications.
- Zhang X et al. Distinctive role of ACVR1 in dentin formation: requirement for dentin thickness in molars and prevention of osteodentin formation in incisors of mice. J Mol Histol 50:43-61 (2019). PubMed: 30519900
- Chung J et al. Protein arginine methyltransferase 5 (PRMT5) promotes survival of lymphoma cells via activation of WNT/ß-catenin and AKT/GSK3ß proliferative signaling. J Biol Chem 294:7692-7710 (2019). PubMed: 30885941
- Brandt R et al. Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium. Nat Commun 10:2919 (2019). PubMed: 31266962
- Ali M et al. Lithium-containing surface pre-reacted glass fillers enhance hDPSC functions and induce reparative dentin formation in a rat pulp capping model through activation of Wnt/ß-catenin signaling. Acta Biomater 96:594-604 (2019). PubMed: 31212112
- Parolia A et al. Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer. Nature 571:413-418 (2019). PubMed: 31243372
Images
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Western blot - Anti-Axin 2 antibody (ab32197)Anti-Axin 2 antibody (ab32197) at 1 µg/ml + AXIN2 partial recombinant tagged protein at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 94 kDaab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab32197)IHC image of ab32197 stained area of normal tissue in a human colorectal cancer FFPE section. Section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 seconds at 125°C. Section was incubated with ab32197 at a dilution of 1:500 for 1h at room temperature and detected using an HRP conjugated polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - Anti-Axin 2 antibody (ab32197)All lanes : Anti-Axin 2 antibody (ab32197) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : Colon (Mouse) Tissue Lysate
Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 4 : LS147T (Colon cancer cells) (Human) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 94 kDa
Observed band size: 94 kDa
Additional bands at: 32 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
