Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP127] to S100 beta - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-S100 beta antibody [SP127] - BSA and Azide free
    See all S100 beta primary antibodies

  • Description

    Rabbit monoclonal [SP127] to S100 beta - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow

  • Immunogen

    Recombinant full length protein corresponding to Human S100 beta.
    Database link: P04271

  • Positive control

    • IHC-P: Human colon, melanoma, kidney, colon, spinal cord, cerebellum, adrenal gland bladder, breast, oesophagus, Mouse cerebrum, and Rat cerebrum tissue; Flow Cyt: A375 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab242388 is the carrier-free version of ab115803. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242388 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)
  • Flow Cytometry - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Flow Cytometry - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Flow Cytometry analysis of A375(Human malignant melanoma epithelial cell) cells labeling S100 beta with purified ab115803 at 1:200 dilution (0.79 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab130748).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human esophagus tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human breast tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human bladder tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human cerebellum tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human spinal cord tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human colon tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human colon tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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