Anti-S100 beta antibody (ab41548)
Key features and details
- Rabbit polyclonal to S100 beta
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-S100 beta antibody
See all S100 beta primary antibodies -
Description
Rabbit polyclonal to S100 beta -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P ZebrafishWB Mouse
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Immunogen
Synthetic peptide corresponding to Rat S100 beta aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab41547) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P ZebrafishWB MouseAll applications RabbitCowHumanPigChinese hamsterApplication Abreviews Notes IHC-P (10) Use at an assay dependent concentration.WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).Notes IHC-P
Use at an assay dependent concentration.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).Application notesIs unsuitable for ICC/IF.Target
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Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. -
Tissue specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. -
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 525716 Cow
- Entrez Gene: 6285 Human
- Entrez Gene: 20203 Mouse
- Entrez Gene: 100009495 Rabbit
- Entrez Gene: 25742 Rat
- Omim: 176990 Human
- SwissProt: P02638 Cow
- SwissProt: P04271 Human
see all -
Alternative names
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (ab41548)This image is courtesy of an Abreview submitted by Carl Hobbs
ab41548 staining S100 beta in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
At this dilution factor, there is very good delineation of astrocyte processes.
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Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab41548)All lanes : Anti-S100 beta antibody (ab41548) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Spinal Cord (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 9 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (ab41548)Image courtesy of Carl Hobbs, King College London, U.K.ab41548 staining S100 beta in Zebrafish body, showing T/S of spinal cord region by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (50)
ab41548 has been referenced in 50 publications.
- Kim JE et al. Single cell and genetic analyses reveal conserved populations and signaling mechanisms of gastrointestinal stromal niches. Nat Commun 11:334 (2020). PubMed: 31953387
- Murphy-Royal C et al. Stress gates an astrocytic energy reservoir to impair synaptic plasticity. Nat Commun 11:2014 (2020). PubMed: 32332733
- Qiu S et al. Decellularized nerve matrix hydrogel and glial-derived neurotrophic factor modifications assisted nerve repair with decellularized nerve matrix scaffolds. J Tissue Eng Regen Med N/A:N/A (2020). PubMed: 32336045
- Salimi H et al. Encephalitic Alphaviruses Exploit Caveola-Mediated Transcytosis at the Blood-Brain Barrier for Central Nervous System Entry. mBio 11:N/A (2020). PubMed: 32047126
- Daun KA et al. Early Maternal and Social Deprivation Expands Neural Stem Cell Population Size and Reduces Hippocampus/Amygdala-Dependent Fear Memory. Front Neurosci 14:22 (2020). PubMed: 32063832
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (ab41548) This image is courtesy of an Abreview submitted by Carl Hobbs
ab41548 staining S100 beta in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
At this dilution factor, there is very good delineation of astrocyte processes.
-
Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab41548)All lanes : Anti-S100 beta antibody (ab41548) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Spinal Cord (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 9 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (ab41548) Image courtesy of Carl Hobbs, King College London, U.K.
ab41548 staining S100 beta in Zebrafish body, showing T/S of spinal cord region by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/10000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
