Anti-Hsc82 antibody (ab30920)
Key features and details
- Rabbit polyclonal to Hsc82
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Hsc82 antibody -
Description
Rabbit polyclonal to Hsc82 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Saccharomyces cerevisiae
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Immunogen
Synthetic peptide corresponding to Saccharomyces cerevisiae Hsc82 aa 650 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab31800) -
Positive control
- Saccharomyces cerevisiae Whole Cell Lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Saccharomyces cerevisiaeApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).Target
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Relevance
The budding yeast Saccharomyces cerevisiae has two genes, HSP82 and HSC82, that encode homologues of HSP90. HSC82 is a molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved in cell cycle control. Cells overexpressing Hsc82 are hypersensitive to various stresses including high concentrations of NaCl. The phenotypes associated with the sensitivity to salt stress of Hsc82-overexpressing cells are similar to those of calcineurin-defective mutants. -
Cellular localization
Cytoplasmic and Mitochondrial -
Database links
- Entrez Gene: 855224 Saccharomyces cerevisiae
- SwissProt: P15108 Saccharomyces cerevisiae
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Alternative names
- 82 kDa heat shock cognate protein antibody
- ATP-dependent molecular chaperone HSC82 antibody
- Heat shock protein Hsp90 constitutive isoform antibody
Images
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Western blot - Anti-Hsc82 antibody (ab30920)Anti-Hsc82 antibody (ab30920) at 1 µg/ml + Saccharomyces cerevisiae Whole Cell Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 81 kDa
Datasheets and documents
References (2)
ab30920 has been referenced in 2 publications.
- Ly N & Cyert MS Calcineurin, the Ca(2+)-dependent phosphatase, regulates Rga2, a Cdc42 GTPase-activating protein, to modulate pheromone signaling. Mol Biol Cell 28:576-586 (2017). WB ; Saccharomyces cerevisiae . PubMed: 28077617
- Weir NR et al. The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane. Elife 6:N/A (2017). WB ; Saccharomyces cerevisiae . PubMed: 28906250
Images
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Western blot - Anti-Hsc82 antibody (ab30920)Anti-Hsc82 antibody (ab30920) at 1 µg/ml + Saccharomyces cerevisiae Whole Cell Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 81 kDa