Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP378Y] to SHIP-1
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
Reacts with: Mouse, Human

Product name

Anti-SHIP-1 antibody [EP378Y]
See all SHIP-1 primary antibodies
Description

Rabbit monoclonal [EP378Y] to SHIP-1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human SHIP-1 aa 1100 to the C-terminus (C terminal). The exact sequence is proprietary.
Positive control
- WB: KM3, Daudi and Ramos cell lysates. IHC-P: Human and mouse spleen tissues. ICC/IF: Jurkat and Raji cells. Flow Cyt: Raji cells. IP: Daudi cell lysate.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP378Y
Isotype

IgG
Research areas

Western blot - Anti-SHIP-1 antibody [EP378Y] (ab45142)

All lanes : Anti-SHIP-1 antibody [EP378Y] (ab45142) at 1/5000 dilution (purified)

Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 133 kDa
Observed band size: 140 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-SHIP-1 antibody [EP378Y] (ab45142)

Anti-SHIP-1 antibody [EP378Y] (ab45142) at 1/1000 dilution (purified) + KM3 cell lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 133 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] (ab45142)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling SHIP-1 with purified ab45142 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] (ab45142)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling SHIP-1 with purified ab45142 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-SHIP-1 antibody [EP378Y] (ab45142)

Immunocytochemistry/Immunofluorescence analysis of Raji cells labelling SHIP-1 with purified ab45142 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.

Control 1: primary antibody (1/150) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Immunocytochemistry/ Immunofluorescence - Anti-SHIP-1 antibody [EP378Y] (ab45142)

Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling SHIP-1 with purified ab45142 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.

Control 1: primary antibody (1/150) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Flow Cytometry - Anti-SHIP-1 antibody [EP378Y] (ab45142)

Flow Cytometry analysis of Raji cells labelling SHIP-1 with purified ab45142 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunoprecipitation - Anti-SHIP-1 antibody [EP378Y] (ab45142)

ab45142 (purified) at 1/20 immunoprecipitating SHIP-1 in Daudi whole cell lysate.

Lane 1 (input): Daudi whole cell lysate (10µg)

Lane 2 (+): ab45142 + Daudi whole cell lysate (10µg).

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45142 in Daudi whole cell lysate.

For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-SHIP-1 antibody [EP378Y] (ab45142)

Anti-SHIP-1 antibody [EP378Y] (ab45142) at 1/50000 dilution (unpurified) + Daudi cell lysate at 10 µg

Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 133 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] (ab45142)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling SHIP-1 with unpurified ab45142 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-SHIP-1 antibody [EP378Y] (ab45142)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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