Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SHIP-1 antibody [EP378Y] (ab45142)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP378Y] to SHIP-1
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SHIP-1 antibody [EP378Y]
    See all SHIP-1 primary antibodies

  • Description

    Rabbit monoclonal [EP378Y] to SHIP-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SHIP-1 aa 1100 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: KM3, Daudi and Ramos cell lysates. IHC-P: Human and mouse spleen tissues. ICC/IF: Jurkat and Raji cells. Flow Cyt: Raji cells. IP: Daudi cell lysate.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Western blot - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    All lanes : Anti-SHIP-1 antibody [EP378Y] (ab45142) at 1/5000 dilution (purified)

    Lane 1 : Daudi cell lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Western blot - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Anti-SHIP-1 antibody [EP378Y] (ab45142) at 1/1000 dilution (purified) + KM3 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] (ab45142)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling SHIP-1 with purified ab45142 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] (ab45142)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling SHIP-1 with purified ab45142 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Immunocytochemistry/ Immunofluorescence - Anti-SHIP-1 antibody [EP378Y] (ab45142)

    Immunocytochemistry/Immunofluorescence analysis of Raji cells labelling SHIP-1 with purified ab45142 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/150) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Immunocytochemistry/ Immunofluorescence - Anti-SHIP-1 antibody [EP378Y] (ab45142)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling SHIP-1 with purified ab45142 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/150) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Flow Cytometry - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Flow Cytometry - Anti-SHIP-1 antibody [EP378Y] (ab45142)

    Flow Cytometry analysis of Raji cells labelling SHIP-1 with purified ab45142 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunoprecipitation - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Immunoprecipitation - Anti-SHIP-1 antibody [EP378Y] (ab45142)

    ab45142 (purified) at 1/20 immunoprecipitating SHIP-1 in Daudi whole cell lysate.

    Lane 1 (input): Daudi whole cell lysate (10µg)

    Lane 2 (+): ab45142 + Daudi whole cell lysate (10µg).

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45142 in Daudi whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Western blot - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Anti-SHIP-1 antibody [EP378Y] (ab45142) at 1/50000 dilution (unpurified) + Daudi cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] (ab45142)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling SHIP-1 with unpurified ab45142 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-SHIP-1 antibody [EP378Y] (ab45142)
    Anti-SHIP-1 antibody [EP378Y] (ab45142)

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