Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-4-1BBL antibody [EPR21995-147] (ab254385)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21995-147] to 4-1BBL
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-4-1BBL antibody [EPR21995-147]
    See all 4-1BBL primary antibodies

  • Description

    Rabbit monoclonal [EPR21995-147] to 4-1BBL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji whole cell lysate. Flow cyt: Raji cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-4-1BBL antibody [EPR21995-147] (ab254385)
    Western blot - Anti-4-1BBL antibody [EPR21995-147] (ab254385)
    All lanes : Anti-4-1BBL antibody [EPR21995-147] (ab254385) at 1/1000 dilution

    Lane 1 : Raji (human Burkitts lymphoma cell line) whole cell lysate at 30 µg
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 60 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 27 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression control: K562 (PMID: 20670353).

    Exposure time:3 minutes.

     

  • Flow Cytometry - Anti-4-1BBL antibody [EPR21995-147] (ab254385)
    Flow Cytometry - Anti-4-1BBL antibody [EPR21995-147] (ab254385)

    Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling 4-1BBL with ab254385 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Anti-4-1BBL antibody [EPR21995-147] (ab254385)
    Anti-4-1BBL antibody [EPR21995-147] (ab254385)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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