Anti-cAMP Protein Kinase Catalytic subunit antibody (ab26322)
Key features and details
- Rabbit polyclonal to cAMP Protein Kinase Catalytic subunit
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Guinea pig, Human, Pig, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-cAMP Protein Kinase Catalytic subunit antibody
See all cAMP Protein Kinase Catalytic subunit primary antibodies -
Description
Rabbit polyclonal to cAMP Protein Kinase Catalytic subunit -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Guinea pig, Human, Pig, Chinese hamster -
Immunogen
Synthetic peptide: PKFKGPGDTSNFDDYEEEC conjugated to KLH, corresponding to amino acids 316-333 of Human cAMP Protein Kinase Catalytic subunit
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-cAMP Protein Kinase Catalytic subunit antibody (ab26322)All lanes : Anti-cAMP Protein Kinase Catalytic subunit antibody (ab26322) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Lysates prepared from CHO-K1 cell line
Lane 3 : Lysate prepared from Mouse brain
Lane 4 : Lysate prepared from Rat brain
Lane 5 : Lysates prepared from Hela cells
Lane 6 : Lysates prepared from mouse 3T3 cells
Lane 7 : Lysates prepared from PC-12 cells
Predicted band size: 40 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cAMP Protein Kinase Catalytic subunit antibody (ab26322)Ab26322 staining human normal testis. Staining is localised to nuclear and cytoplasmic compartments.
Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH6 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplificati -
Immunocytochemistry/ Immunofluorescence - Anti-cAMP Protein Kinase Catalytic subunit antibody (ab26322)ICC/IF image of ab26322 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26322, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
