Anti-NCR1 antibody [EPR22403-57] - BSA and Azide free (ab254209)
![Anti-NCR1 antibody [EPR22403-57] - BSA and Azide free (ab254209)](https://www.abcam.com/ps/products/254/ab254209/Images/ab254209-2-224703IHC2.jpg "Anti-NCR1 antibody [EPR22403-57] - BSA and Azide free (ab254209)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22403-57] to NCR1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-NCR1 antibody [EPR22403-57] - BSA and Azide free
See all NCR1 primary antibodies -
Description
Rabbit monoclonal [EPR22403-57] to NCR1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NK-92 whole cell lysate. IHC-P: Human tonsil and NK/T cell lymphoma.
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General notes
ab254209 is the carrier-free version of ab224703. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab254209 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22403-57 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] - BSA and Azide free (ab254209)
Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue labeling NCR1 with ab224703 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human NK/T-cell lymphoma (PMID: 24225754) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224703).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] - BSA and Azide free (ab254209)](https://www.abcam.com/ps/products/254/ab254209/Images/ab254209-1-224703IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] - BSA and Azide free (ab254209)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling NCR1 with ab224703 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in marginal zone of human tonsil (PMID: 24225754) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224703).
-
![Anti-NCR1 antibody [EPR22403-57] - BSA and Azide free (ab254209)](https://www.abcam.com/ps/products/254/ab254209/Images/ab254209-3-benefits-of-recombinant-antibodies.png)
Anti-NCR1 antibody [EPR22403-57] - BSA and Azide free (ab254209)
