Anti-NOX2/gp91phox antibody [54.1] (ab80897)
![Anti-NOX2/gp91phox antibody [54.1] (ab80897)](https://www.abcam.com/ps/products/80/ab80897/Images/ab80897-96144-anti-nox2-gp91phox-antibody-541-western-blot.jpg "Anti-NOX2/gp91phox antibody [54.1] (ab80897)")
Key features and details
- Mouse monoclonal [54.1] to NOX2/gp91phox
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NOX2/gp91phox antibody [54.1]
See all NOX2/gp91phox primary antibodies -
Description
Mouse monoclonal [54.1] to NOX2/gp91phox -
Host species
Mouse -
Specificity
This antibody is specific for NOX2/gp91phox 382-PKIAVDGP-389. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue/ cell preparation (Human): Partially Purified human neutrophil flavocytochrome b (heparin Ultrogel/lectin affinity purification in Triton X-100).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
54.1 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-NOX2/gp91phox antibody [54.1] (ab80897)All lanes : Anti-NOX2/gp91phox antibody [54.1] (ab80897) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 29 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-NOX2/gp91phox antibody [54.1] (ab80897)](https://www.abcam.com/ps/products/80/ab80897/Images/ab80897-96143-anti-nox2-gp91phox-antibody-541-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NOX2/gp91phox antibody [54.1] (ab80897)ICC/IF image of ab80897 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80897, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [54.1] (ab80897)](https://www.abcam.com/ps/products/80/ab80897/Images/ab80897-96142-anti-nox2-gp91phox-antibody-541-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [54.1] (ab80897)IHC image of ab80897 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
