Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6991] to NOX2/gp91phox
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NOX2/gp91phox antibody [EPR6991]
    See all NOX2/gp91phox primary antibodies

  • Description

    Rabbit monoclonal [EPR6991] to NOX2/gp91phox

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NOX2/gp91phox aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: MCF-7, HepG2 and Caco-2 cell lysates, mouse and rat brain and spleen tissue lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
    Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
    All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 65 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
    Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
    All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)

    Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
    Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 65 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
    Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
    All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/1000 dilution (unpurified)

    Lane 1 : MCF-7 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 65 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
    OI-RD Scanning - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
    Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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