Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

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Anti-CRTAM antibody [EPR23786-12] (ab272723)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23786-12] to CRTAM
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-CRTAM antibody [EPR23786-12]
See all CRTAM primary antibodies
Description

Rabbit monoclonal [EPR23786-12] to CRTAM
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse thymus tissue lysate; Rat cerebellum tissue lysate; Human PBMC (untreated and treated with 1ng/mL phorbol-12-myristate-13-acetate and 0.2 µM ionomycin for 5 hours) whole cell lysates. Flow Cyt: Human PBMCs.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23786-12
Isotype

IgG
Research areas

Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] (ab272723)

Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) (treated with 1 ng/mL PMA and 0.2 μM Ionomycin for 5h) labelling CRTAM with ab272723 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG or ab272723. Then stained with anti-CD8 alpha conjugated to Pacific blue.

Gated on viable cells.
Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)

All lanes : Anti-CRTAM antibody [EPR23786-12] (ab272723) at 1/1000 dilution

Lane 1 : Untreated human peripheral blood mononuclear cell (PBMC) whole cell lysate with NFDM/TBST
Lane 2 : Human PBMC treated with 1ng/mL phorbol-12-myristate-13-acetate (PMA) (ab120297) and 0.2 µM ionomycin (ab120116) for 5 hours, whole cell lysate with NFDM/TBST

Lysates/proteins at 20 µg per lane.

Blocking peptides at 5 % per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody)

Predicted band size: 45 kDa
Observed band size: 70/85 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

The molecular weight observed is consistent with what has been described in the literature (PMID:10811014, 23637756, 16300832) .
Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)

All lanes : Anti-CRTAM antibody [EPR23786-12] (ab272723) at 1/1000 dilution

Lane 1 : Mouse thymus tissue lysate with NFDM/TBST
Lane 2 : Rat cerebellum tissue lysate with NFDM/TBST

Lysates/proteins at 40 µg per lane.

Blocking peptides at 5 % per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 45 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

The molecular weight observed is consistent with what has been described in the literature (PMID:10811014, 23637756, 16300832).
Anti-CRTAM antibody [EPR23786-12] (ab272723)

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