Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRTAM antibody [EPR23786-12] (ab272723)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23786-12] to CRTAM
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CRTAM antibody [EPR23786-12]
    See all CRTAM primary antibodies

  • Description

    Rabbit monoclonal [EPR23786-12] to CRTAM

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse thymus tissue lysate; Rat cerebellum tissue lysate; Human PBMC (untreated and treated with 1ng/mL phorbol-12-myristate-13-acetate and 0.2 µM ionomycin for 5 hours) whole cell lysates. Flow Cyt: Human PBMCs.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23786-12

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] (ab272723)
    Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] (ab272723)

    Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) (treated with 1 ng/mL PMA and 0.2 μM Ionomycin for 5h) labelling CRTAM with ab272723 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG or ab272723. Then stained with anti-CD8 alpha conjugated to Pacific blue.

    Gated on viable cells.

  • Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
    Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
    All lanes : Anti-CRTAM antibody [EPR23786-12] (ab272723) at 1/1000 dilution

    Lane 1 : Untreated human peripheral blood mononuclear cell (PBMC) whole cell lysate with NFDM/TBST
    Lane 2 : Human PBMC treated with 1ng/mL phorbol-12-myristate-13-acetate (PMA) (ab120297) and 0.2 µM ionomycin (ab120116) for 5 hours, whole cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody)

    Predicted band size: 45 kDa
    Observed band size: 70/85 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The molecular weight observed is consistent with what has been described in the literature (PMID:10811014, 23637756, 16300832) .

  • Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
    Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
    All lanes : Anti-CRTAM antibody [EPR23786-12] (ab272723) at 1/1000 dilution

    Lane 1 : Mouse thymus tissue lysate with NFDM/TBST
    Lane 2 : Rat cerebellum tissue lysate with NFDM/TBST

    Lysates/proteins at 40 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 45 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The molecular weight observed is consistent with what has been described in the literature (PMID:10811014, 23637756, 16300832).

  • Anti-CRTAM antibody [EPR23786-12] (ab272723)
    Anti-CRTAM antibody [EPR23786-12] (ab272723)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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