Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin beta 1 antibody [EPR16896] - BSA and Azide free (ab250101)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16896] to Integrin beta 1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Integrin beta 1 antibody [EPR16896] - BSA and Azide free
    See all Integrin beta 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16896] to Integrin beta 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HCT116 and HAP1 cell lysates.

  • General notes

    ab250101 is the carrier-free version of ab179472. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250101 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] - BSA and Azide free (ab250101)
    Western blot - Anti-Integrin beta 1 antibody [EPR16896] - BSA and Azide free (ab250101)
    All lanes : Anti-Integrin beta 1 antibody [EPR16896] (ab179472) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ITGB1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 88 kDa
    Observed band size: 140-150 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab179472).

      Lanes 1- 2: Merged signal (red and green). Green - ab179472 observed at 140-150 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab179472 was shown to react with ITGB1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255354 (knockout cell lysate ab263847) was used. Wild-type HeLa and ITGB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab179472 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] - BSA and Azide free (ab250101)
    Western blot - Anti-Integrin beta 1 antibody [EPR16896] - BSA and Azide free (ab250101)
    All lanes : Anti-Integrin beta 1 antibody [EPR16896] (ab179472) at 1/1000 dilution

    Lane 1 : Wild-type HCT116 cell lysate
    Lane 2 : ITGB1 knockout HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 88 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab179472).

    Lanes 1 - 2: Merged signal (red and green). Green - ab179472 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab179472 was shown to react with Integrin beta 1 in HCT 116 wild-type cells in western blot with loss of signal observed in ITGB1 knockout cell line ab273724 (ITGB1 knockout cell lysate ab275251). HCT 116 wild-type and ITGB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab179472 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Integrin beta 1 antibody [EPR16896] - BSA and Azide free (ab250101)
    Western blot - Anti-Integrin beta 1 antibody [EPR16896] - BSA and Azide free (ab250101)
    All lanes : Anti-Integrin beta 1 antibody [EPR16896] (ab179472) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : Integrin beta 1 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 88 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab179472).

    Lanes 1 - 2: Merged signal (red and green). Green - ab179472 observed at 125 kDa. Red - loading control, ab9484, observed at 37 kDa.


    ab179472 was shown to specifically react with Integrin beta 1 in wild-type HAP1 cells as signal was lost in Integrin beta 1 knockout cells. Wild-type and Integrin beta 1 knockout samples were subjected to SDS-PAGE. Ab179472 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at  1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Integrin beta 1 antibody [EPR16896] - BSA and Azide free (ab250101)
    Anti-Integrin beta 1 antibody [EPR16896] - BSA and Azide free (ab250101)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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