Anti-IgD antibody [11-26] - BSA and Azide free (ab235126)
Key features and details
- Rat monoclonal [11-26] to IgD - BSA and Azide free
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-IgD antibody [11-26] - BSA and Azide free
See all IgD primary antibodies -
Description
Rat monoclonal [11-26] to IgD - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length protein corresponding to Mouse IgD.
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Positive control
- IHC-Fr: Mouse spleen tissue. Flow Cyt: C57/BL6 splenocytes.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
11-26 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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C57BL/6 mouse splenocytes stained with ab235126 (right) or rat IgG2aκ (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 10% mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab235126) or rat IgG2aκ Isotype (ab18450) (1x106 in 100µl at 0.1 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°C.The cells were simultaneously stained with CD19 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
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IHC image of IgD staining in a section of frozen normal mouse spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab235126 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150167 (Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 647) preabsorbed) (shown in red) and DAPI (staining nuclear DNA)(shown in blue) for 1 hour at room temperature . The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab235126.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from Charles River.