Biotin Anti-IgD antibody [RM123] (ab224182)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [RM123] to IgD
- Suitable for: Sandwich ELISA, ELISA, IHC
- Reacts with: Human
- Conjugation: Biotin
Overview
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Product name
Biotin Anti-IgD antibody [RM123]
See all IgD primary antibodies -
Description
Biotin Rabbit monoclonal [RM123] to IgD -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
ab224182 reacts to human IgD. No cross reactivity with human IgG, IgM, IgA, or IgE. -
Tested applications
Suitable for: Sandwich ELISA, ELISA, IHCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human IgD aa 1 to the C-terminus.
Database link: P01880
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab224182 was purified from an animal origin–free culture supernatant . -
Clonality
Monoclonal -
Clone number
RM123 -
Isotype
IgG -
Research areas
Images
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Sandwich ELISA using ab224182 as the capture antibody (100 ng/well), and ab193185 as the detection antibody, followed by an alkaline phosphatase-conjugated streptavidin.
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A titre ELISA using ab224182. The plate was coated with different amounts of human IgD from plasma. A serial dilution of ab224182 was used as primary antibody. An alkaline phosphatase-conjugated Anti-Rabbit IgG was used as secondary antibody.
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ELISA of human immunoglobulins shows that ab224182 reacts to IgD from human plasma and IgDκ from human melanoma. No cross reactivity with human IgG, IgM, IgA or IgE. The plate was coated with 50 ng/well of the different immunoglobulins. 200 ng/ml, 50 ng/ml or 10 ng/ml of ab224182 was used as the primary antibody. An alkaline phosphatase-conjugated Anti-Rabbit IgG was used as secondary antibody.
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Human lymphoid tissue stained for IgD with ab224182 (2 μg/ml) in immunohistochemical analysis.
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