Antibodies, Proteins, Kits and Reagents for Life Science

Biotin Anti-IgD antibody [RM123] (ab224182)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Biotin Rabbit monoclonal [RM123] to IgD
Suitable for: Sandwich ELISA, ELISA, IHC
Reacts with: Human
Conjugation: Biotin

Product name

Biotin Anti-IgD antibody [RM123]
See all IgD primary antibodies
Description

Biotin Rabbit monoclonal [RM123] to IgD
Host species

Rabbit
Conjugation

Biotin
Specificity

ab224182 reacts to human IgD. No cross reactivity with human IgG, IgM, IgA, or IgE.
Tested applications

Suitable for: Sandwich ELISA, ELISA, IHCmore details
Species reactivity

Reacts with: Human
Immunogen

Full length native protein (purified) corresponding to Human IgD aa 1 to the C-terminus.
Database link: P01880

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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

ab224182 was purified from an animal origin–free culture supernatant .
Clonality

Monoclonal
Clone number

RM123
Isotype

IgG
Research areas

Sandwich ELISA - Biotin Anti-IgD antibody [RM123] (ab224182)

Sandwich ELISA using ab224182 as the capture antibody (100 ng/well), and ab193185 as the detection antibody, followed by an alkaline phosphatase-conjugated streptavidin.
ELISA - Biotin Anti-IgD antibody [RM123] (ab224182)

A titre ELISA using ab224182. The plate was coated with different amounts of human IgD from plasma. A serial dilution of ab224182 was used as primary antibody. An alkaline phosphatase-conjugated Anti-Rabbit IgG was used as secondary antibody.
ELISA - Biotin Anti-IgD antibody [RM123] (ab224182)

ELISA of human immunoglobulins shows that ab224182 reacts to IgD from human plasma and IgDκ from human melanoma. No cross reactivity with human IgG, IgM, IgA or IgE. The plate was coated with 50 ng/well of the different immunoglobulins. 200 ng/ml, 50 ng/ml or 10 ng/ml of ab224182 was used as the primary antibody. An alkaline phosphatase-conjugated Anti-Rabbit IgG was used as secondary antibody.
Immunohistochemistry - Biotin Anti-IgD antibody [RM123] (ab224182)

Human lymphoid tissue stained for IgD with ab224182 (2 μg/ml) in immunohistochemical analysis.
Biotin Anti-IgD antibody [RM123] (ab224182)

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