Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [IA6-2] to IgD - BSA and Azide free
  • Suitable for: Flow Cyt, WB, Indirect ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-IgD antibody [IA6-2] - BSA and Azide free
    See all IgD primary antibodies

  • Description

    Mouse monoclonal [IA6-2] to IgD - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WB, Indirect ELISAmore details
    Unsuitable for: IHC-Fr or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    The details of the immunogen for this antibody are not available.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human plasma and serum lysate. Flow Cyt: Human peripheral blood mononuclear cell (PBMC).

  • General notes

    ab269972 is the carrier-free version of ab235125.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
    Western blot - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
    All lanes : Anti-IGHD antibody [IA6-2] (ab235125) at 1.032 µg/ml

    Lane 1 : Human plasma lyaste
    Lane 2 : Human serum lyaste

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 42 kDa


    Exposure time: 8 seconds


    This antibody recognize the heavy chain (~60 kDa) of IgD.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235125).

  • Flow Cytometry - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
    Flow Cytometry - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling IGHD with ab235125 at 1/500 dilution (2.064µg/ml) (Right panel) compared with a mouse monoclonal IgG isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab235125 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235125).

  • ELISA - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
    ELISA - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)

    ELISA - Anti-IGHD antibody [IA6-2] (ab235125).

    ab235125 used at 0-4000 ng/ml.

    Antigen (Human IGD) used at 1000 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235125).

  • Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
    Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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