Anti-CD11c antibody [N418] - BSA and Azide free (ab233173)
Key features and details
- Armenian hamster monoclonal [N418] to CD11c - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CD11c antibody [N418] - BSA and Azide free
See all CD11c primary antibodies -
Description
Armenian hamster monoclonal [N418] to CD11c - BSA and Azide free -
Host species
Armenian hamster -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD11c.
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Positive control
- Flow Cyt: Murine alveolar cells. Mouse splenic cells
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General notes
ab233173 is the PBS only version of ab33483.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N418 -
Myeloma
Sp2/0 -
Isotype
IgG -
Research areas
Images
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Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labeling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000.
Gating strategy was against CD45+, C11b+, GR1-cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33483).
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Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.
Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33483).