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Anti-CD11c antibody [N418] - BSA and Azide free (ab233173)

Key features and details

  • Armenian hamster monoclonal [N418] to CD11c - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Isotype: IgG

Overview

  • Product name

    Anti-CD11c antibody [N418] - BSA and Azide free
    See all CD11c primary antibodies

  • Description

    Armenian hamster monoclonal [N418] to CD11c - BSA and Azide free

  • Host species

    Armenian hamster

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD11c.

  • Positive control

    • Flow Cyt: Murine alveolar cells. Mouse splenic cells

  • General notes

    ab233173 is the PBS only version of ab33483.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.

Properties

Images

  • Flow Cytometry - Anti-CD11c antibody [N418] - BSA and Azide free (ab233173)
    Flow Cytometry - Anti-CD11c antibody [N418] - BSA and Azide free (ab233173)

    Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labeling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000.

    Gating strategy was against CD45+, C11b+, GR1-cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33483).

  • Flow Cytometry - Anti-CD11c antibody [N418] - BSA and Azide free (ab233173)
    Flow Cytometry - Anti-CD11c antibody [N418] - BSA and Azide free (ab233173)

    Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488)  preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.

    Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33483).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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