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Immunology Immunoglobulins Heavy Chain IgD

Anti-IGHD antibody [IA6-2] (ab235125)

Anti-IGHD antibody [IA6-2] (ab235125)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [IA6-2] to IGHD
  • Suitable for: Flow Cyt, ELISA, WB
  • Reacts with: Human, Recombinant fragment

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Overview

  • Product name

    Anti-IGHD antibody [IA6-2]
    See all IGHD primary antibodies
  • Description

    Mouse monoclonal [IA6-2] to IGHD
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    The details of the immunogen for this antibody are not available.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human plasma and serum lysate. Flow Cyt: Human peripheral blood mononuclear cell (PBMC).
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    IA6-2
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgD

Images

  • Western blot - Anti-IGHD antibody [IA6-2] (ab235125)
    Western blot - Anti-IGHD antibody [IA6-2] (ab235125)
    All lanes : Anti-IGHD antibody [IA6-2] (ab235125) at 1.032 µg/ml

    Lane 1 : Human plasma lyaste
    Lane 2 : Human serum lyaste

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 42 kDa


    Exposure time: 8 seconds


    This antibody recognize the heavy chain (~60 kDa) of IgD.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-IGHD antibody [IA6-2] (ab235125)
    Flow Cytometry - Anti-IGHD antibody [IA6-2] (ab235125)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling IGHD with ab235125 at 1/500 dilution (2.064µg/ml) (Right panel) compared with a mouse monoclonal IgG isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab235125 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.

  • ELISA - Anti-IGHD antibody [IA6-2] (ab235125)
    ELISA - Anti-IGHD antibody [IA6-2] (ab235125)

    ELISA - Anti-IGHD antibody [IA6-2] (ab235125).

    ab235125 used at 0-4000 ng/ml.

    Antigen (Human IGD) used at 1000 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

  • Anti-IGHD antibody [IA6-2] (ab235125)
    Anti-IGHD antibody [IA6-2] (ab235125)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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