Anti-IGHD antibody [IA6-2] (ab235125)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [IA6-2] to IGHD
- Suitable for: Flow Cyt, ELISA, WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-IGHD antibody [IA6-2]
See all IGHD primary antibodies -
Description
Mouse monoclonal [IA6-2] to IGHD -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentFlow Cyt HumanWB Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- WB: Human plasma and serum lysate. Flow Cyt: Human peripheral blood mononuclear cell (PBMC).
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
IA6-2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-IGHD antibody [IA6-2] (ab235125) at 1.032 µg/ml
Lane 1 : Human plasma lyaste
Lane 2 : Human serum lyaste
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 42 kDa
Exposure time: 8 secondsThis antibody recognize the heavy chain (~60 kDa) of IgD.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling IGHD with ab235125 at 1/500 dilution (2.064µg/ml) (Right panel) compared with a mouse monoclonal IgG isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab235125 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.
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ELISA - Anti-IGHD antibody [IA6-2] (ab235125).
ab235125 used at 0-4000 ng/ml.
Antigen (Human IGD) used at 1000 ng/ml.
Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.
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