Rabbit F(ab')2 Anti-Sheep IgG H&L (Alkaline Phosphatase) (ab136765)
Key features and details
- Rabbit F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (AP)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-Fr, IHC-P, Flow Cyt, ICC, WB, ELISA
Overview
-
Product name
Rabbit F(ab')2 Anti-Sheep IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Description
Rabbit F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (AP) -
Host species
Rabbit -
Target species
Sheep -
Tested applications
Suitable for: IHC-Fr, IHC-P, Flow Cyt, ICC, WB, ELISAmore details -
Immunogen
Highly purified whole immunoglobulin from Sheep.
-
Conjugation
Alkaline Phosphatase
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 48.9% Tris buffered saline, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136765 is purified by affinity chromatography and is thus particularly specific. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Preparation of Substrate Solutions:
ELISA : 1M diethanolamin, 0.5mM MgCl2, 15mM pNPP, pH 9.8. Incubate for 15 minutes at 37°C. Stop the reaction with V/5 K2HP04 2M. Read at 405 nm.
Immunoblot : 34 µl of BCIP (5bromo-4chloroindolylphosphate) at 50mg/ml in DMF + 44 µl NBT (NitroBlue Tetrazolium) at 75 mg/ml in DMF 70% + 10ml substrate buffer (0.1M TRIS/HCI, 0.5M NaCl, 0.05M MgCl2, pH 9.5). -
Research areas
