Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHC
Overview
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Product name
Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.. -
Tested applications
Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
Minimal
cross-reactivity
Cow, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Rabbit IgG F(ab’)2 fragment
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6112, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
