Anti-Glutamine Synthetase antibody (ab93439)
Key features and details
- Rabbit polyclonal to Glutamine Synthetase
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies -
Description
Rabbit polyclonal to Glutamine Synthetase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Glutamine Synthetase aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab103264)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications ChickenCowDogHumanPigZebrafishCynomolgus monkeyChinese hamsterApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).Target
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Function
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. -
Involvement in disease
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. -
Sequence similarities
Belongs to the glutamine synthetase family. -
Developmental stage
Expressed during early fetal stages. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 396489 Chicken
- Entrez Gene: 281199 Cow
- Entrez Gene: 2752 Human
- Entrez Gene: 14645 Mouse
- Entrez Gene: 396944 Pig
- Entrez Gene: 24957 Rat
- Omim: 138290 Human
- SwissProt: P16580 Chicken
see all -
Alternative names
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
see all
Images
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Western blot - Anti-Glutamine Synthetase antibody (ab93439)All lanes : Anti-Glutamine Synthetase antibody (ab93439) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Cerebellum (Mouse) Tissue Lysate
Lane 4 : Cerebellum (Rat) Tissue Lysate
Lane 5 : Kidney (Mouse) Tissue Lysate
Lane 6 : Kidney (Rat) Tissue Lysate
Lane 7 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Protocols
Datasheets and documents
References (4)
ab93439 has been referenced in 4 publications.
- Mitra S et al. Dual regulation of lin28a by Myc is necessary during zebrafish retina regeneration. J Cell Biol 218:489-507 (2019). PubMed: 30606747
- Mitra S et al. Histone Deacetylase-Mediated Müller Glia Reprogramming through Her4.1-Lin28a Axis Is Essential for Retina Regeneration in Zebrafish. iScience 7:68-84 (2018). PubMed: 30267687
- Morales I et al. Striatal astrocytes engulf dopaminergic debris in Parkinson's disease: A study in an animal model. PLoS One 12:e0185989 (2017). PubMed: 29028815
- Morales I et al. The astrocytic response to the dopaminergic denervation of the striatum. J Neurochem 139:81-95 (2016). PubMed: 27230040
Images
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Western blot - Anti-Glutamine Synthetase antibody (ab93439)All lanes : Anti-Glutamine Synthetase antibody (ab93439) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Cerebellum (Mouse) Tissue Lysate
Lane 4 : Cerebellum (Rat) Tissue Lysate
Lane 5 : Kidney (Mouse) Tissue Lysate
Lane 6 : Kidney (Rat) Tissue Lysate
Lane 7 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
