Anti-Glutamine Synthetase antibody (ab228590)
Key features and details
- Rabbit polyclonal to Glutamine Synthetase
- Suitable for: IHC-P, WB, IP, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Glutamine Synthetase antibody
See all Glutamine Synthetase primary antibodies -
Description
Rabbit polyclonal to Glutamine Synthetase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-Fr MouseIHC-P MouseHumanIP HumanWB MouseRatHuman
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Immunogen
Recombinant full length protein corresponding to Human Glutamine Synthetase aa 1 to the C-terminus.
Database link: P15104 -
Positive control
- WB: U-87 MG, HEK-293T, SK-N-SH, IMR32, SK-N-AS whole cell extracts; Mouse eye and brain tissue extracts; Rat brain tissue extract. ICC/IF: DIV9 rat E18 primary cortical neurons. IHC-P: H441 xenograft tissue; Adult mouse retina tissue. IHC-Fr: Adult mouse cerebellum tissue. IP: IMR32 whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody (ab228590)
Paraffin-embedded adult mouse retina tissue stained for Glutamine Synthetase (green) using ab228590 at 1/250 dilution in immunohistochemical analysis.
Nuclear counter stain: Fluoroshield with DAPI (blue). beta Tubulin 3/Tuj1 was stained by an anti-beta Tubulin 3/Tuj1 antibody (red) at 1/250 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamine Synthetase antibody (ab228590)Paraffin-embedded H441 (human) xenograft tissue stained for Glutamine Synthetase using ab228590 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Glutamine Synthetase antibody (ab228590)All lanes : Anti-Glutamine Synthetase antibody (ab228590) at 1/5000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Frozen sections) - Anti-Glutamine Synthetase antibody (ab228590)Frozen sections of adult mouse cerebellum tissue stained for Glutamine Synthetase (green) using ab228590 at 1/250 dilution in immunohistochemical analysis.
Nuclear counter stain: Fluoroshield with DAPI (blue). beta Tubulin 3/Tuj1 was stained by an anti-beta Tubulin 3/Tuj1 antibody (red) at 1/500 dilution.
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Western blot - Anti-Glutamine Synthetase antibody (ab228590)Anti-Glutamine Synthetase antibody (ab228590) at 1/20000 dilution + Mouse eye tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Glutamine Synthetase antibody (ab228590)Paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for Glutamine Synthetase (green) using ab228590 at 1/500 dilution in ICF/IF.
Nuclear counter stain: Fluoroshield with DAPI (blue). beta Tubulin 3/Tuj1 was stained by an anti-beta Tubulin 3/Tuj1 antibody (red) at 1/500 dilution.
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Immunoprecipitation - Anti-Glutamine Synthetase antibody (ab228590)Glutamine Synthetase was immunoprecipitated from IMR32 whole cell extract with 5 µg of ab228590. Western blot was performed from the immunoprecipitate using ab228590. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab228590 in IMR32 whole cell extract.
Lane 2: ab228590 IP in IMR32 whole cell extract. -
Western blot - Anti-Glutamine Synthetase antibody (ab228590)Anti-Glutamine Synthetase antibody (ab228590) at 1/50000 dilution + Mouse brain tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Western blot - Anti-Glutamine Synthetase antibody (ab228590)Anti-Glutamine Synthetase antibody (ab228590) at 1/50000 dilution + Rat brain tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Western blot - Anti-Glutamine Synthetase antibody (ab228590)All lanes : Anti-Glutamine Synthetase antibody (ab228590) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract.
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Glutamine Synthetase shRNA, whole cell extract.
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
