Anti-EAAT1 antibody (ab41751)
Key features and details
- Rabbit polyclonal to EAAT1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-EAAT1 antibody
See all EAAT1 primary antibodies -
Description
Rabbit polyclonal to EAAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-FoFr -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human EAAT1.
Read Abcam's proprietary immunogen policy (Peptide available as ab42682.)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab41751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa). ICC/IF Use a concentration of 5 µg/ml. Application notesIs unsuitable for IHC-FoFr.Target
-
Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. -
Tissue specificity
Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. -
Involvement in disease
Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia. -
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6507 Human
- Entrez Gene: 20512 Mouse
- Entrez Gene: 29483 Rat
- Omim: 600111 Human
- SwissProt: P43003 Human
- SwissProt: P56564 Mouse
- SwissProt: P24942 Rat
- Unigene: 481918 Human
see all -
Alternative names
- EA6 antibody
- EAA1_HUMAN antibody
- EAAT1 antibody
see all
Images
-
Western blot - Anti-EAAT1 antibody (ab41751)Anti-EAAT1 antibody (ab41751) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody (ab41751)ICC/IF image of ab41751 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41751, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
Protocols
References (5)
ab41751 has been referenced in 5 publications.
- Mohammadshirazi A et al. Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model. J Cell Physiol N/A:N/A (2019). PubMed: 31004353
- Greferath U et al. Inner retinal change in a novel rd1-FTL mouse model of retinal degeneration. Front Cell Neurosci 9:293 (2015). PubMed: 26283925
- Russo R et al. Impairment of neuronal glutamate uptake and modulation of the glutamate transporter GLT-1 induced by retinal ischemia. PLoS One 8:e69250 (2013). IHC . PubMed: 23936321
- Honsa P et al. Polydendrocytes display large lineage plasticity following focal cerebral ischemia. PLoS One 7:e36816 (2012). IHC-Fr ; Mouse . PubMed: 22590616
- Helms HC et al. In vitro evidence for the brain glutamate efflux hypothesis: brain endothelial cells cocultured with astrocytes display a polarized brain-to-blood transport of glutamate. Glia 60:882-93 (2012). ICC/IF . PubMed: 22392649
Images
-
Western blot - Anti-EAAT1 antibody (ab41751)Anti-EAAT1 antibody (ab41751) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
-
Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody (ab41751)ICC/IF image of ab41751 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41751, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
