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Neuroscience Neurotransmitter Transporters Glutamate

Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557)

Price and availability

311 587 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12685] to EAAT1 - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-EAAT1 antibody [EPR12685] - N-terminal
    See all EAAT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12685] to EAAT1 - N-terminal
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human EAAT1 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: P43003

  • Positive control

    • Human cerebellum and Human fetal brain lysates; Human cerebellum and glioma tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12685
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Aspartate
    • Neuroscience
    • Neurotransmitter
    • Transporters
    • Glutamate

Images

  • Western blot - Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557)
    Western blot - Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557)
    All lanes : Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 59 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebellum tissue, labeling EAAT1 using ab176557 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human glioma tissue, labeling EAAT1 using ab176557 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557)
    Anti-EAAT1 antibody [EPR12685] - N-terminal (ab176557)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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