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Neuroscience Neurotransmitter Transporters Glutamate

Anti-EAAT1 antibody (ab235203)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-EAAT1 antibody (ab235203)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Chicken polyclonal to EAAT1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgY

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Overview

  • Product name

    Anti-EAAT1 antibody
    See all EAAT1 primary antibodies
  • Description

    Chicken polyclonal to EAAT1
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat pup PND2-6 - minimal cortex brain lysate. ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    IgY fraction
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Aspartate
    • Neuroscience
    • Neurotransmitter
    • Transporters
    • Glutamate

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody (ab235203)
    Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody (ab235203)

    4% PFA fixed, 0.15% Triton X-100 permeabilized post-natal rat pup (PND1) heterogeneous brain cells* labeling EAAT1 using ab235203 at 15 µg/ml, O/N at +4°C (green) followed by Goat Anti-Chicken IgG DyLight™ 488 conjugated preadsorbed at 5 µg/ml for 1 h at RT, in ICC/IF. Nuclear probe is visualized in blue (DAPI).

    *UniprotID Expression Tissue Specificity and Subcellular Localization, as well as additional information found in Human Protein Atlas.

    Expected localization:  Cell membrane (mitochondria, nucleus)
    Observed localization: punctate staining indicates mitochondrial detection**

    **Staining as confirmed in Gensat for Slc1a3 (high affinity glial glutamate transporter): http://www.gensat.org/imagenavigator.jsp?imageID=10581

  • Western blot - Anti-EAAT1 antibody (ab235203)
    Western blot - Anti-EAAT1 antibody (ab235203)
    All lanes : Anti-EAAT1 antibody (ab235203) at 1/500 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 20 µg
    Lane 2 : Adult mouse whole liver lysate at 20 µg
    Lane 3 : Rat pup PND2-6 - minimal cortex brain lysate* at 20 µg
    Lane 4 : Rat pup PND2-6 - minimal cortex brain lysate* at 40 µg

    Secondary
    All lanes : Goat Anti-Chicken IgY H&L (HRP) preadsorbed (ab7118) at 1/40000 dilution

    Predicted band size: 59 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    *UniprotID Expression Tissue Specificity: Detected in brain and cerebellum (PubMed:1279699, PubMed:8387171). Both isoform EAAT1/GLAST-1 and EAAT1/GLAST-1A are expressed in bone and brain (PubMed:11086157). In brain isoform EAAT1/GLAST-1 is highly enriched in the Purkinje cell layer in cerebellum (PubMed:11086157). 

    Minimal cortex defined: minimal cortex brain samples have had a portion of the cortex removed, with 1/3 cortex, cerebellum and other brain components present in the final lysate.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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