Anti-EAAT1 antibody (ab235203)
Key features and details
- Chicken polyclonal to EAAT1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
Overview
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Product name
Anti-EAAT1 antibody
See all EAAT1 primary antibodies -
Description
Chicken polyclonal to EAAT1 -
Host species
Chicken -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat pup PND2-6 - minimal cortex brain lysate. ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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4% PFA fixed, 0.15% Triton X-100 permeabilized post-natal rat pup (PND1) heterogeneous brain cells* labeling EAAT1 using ab235203 at 15 µg/ml, O/N at +4°C (green) followed by Goat Anti-Chicken IgG DyLight™ 488 conjugated preadsorbed at 5 µg/ml for 1 h at RT, in ICC/IF. Nuclear probe is visualized in blue (DAPI).
*UniprotID Expression Tissue Specificity and Subcellular Localization, as well as additional information found in Human Protein Atlas.
Expected localization: Cell membrane (mitochondria, nucleus)
Observed localization: punctate staining indicates mitochondrial detection****Staining as confirmed in Gensat for Slc1a3 (high affinity glial glutamate transporter): http://www.gensat.org/imagenavigator.jsp?imageID=10581
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All lanes : Anti-EAAT1 antibody (ab235203) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 20 µg
Lane 2 : Adult mouse whole liver lysate at 20 µg
Lane 3 : Rat pup PND2-6 - minimal cortex brain lysate* at 20 µg
Lane 4 : Rat pup PND2-6 - minimal cortex brain lysate* at 40 µg
Secondary
All lanes : Goat Anti-Chicken IgY H&L (HRP) preadsorbed (ab7118) at 1/40000 dilution
Predicted band size: 59 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 60 seconds*UniprotID Expression Tissue Specificity: Detected in brain and cerebellum (PubMed:1279699, PubMed:8387171). Both isoform EAAT1/GLAST-1 and EAAT1/GLAST-1A are expressed in bone and brain (PubMed:11086157). In brain isoform EAAT1/GLAST-1 is highly enriched in the Purkinje cell layer in cerebellum (PubMed:11086157).
Minimal cortex defined: minimal cortex brain samples have had a portion of the cortex removed, with 1/3 cortex, cerebellum and other brain components present in the final lysate.