Anti-PARIS antibody (ab130867)
Key features and details
- Rabbit polyclonal to PARIS
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-PARIS antibody -
Description
Rabbit polyclonal to PARIS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Horse, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human PARIS aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab157686) -
Positive control
- This antibody gave a positive signal in Mouse Brain tissue lysate. IHC-P: Rat brain FFPE tissue sections
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab130867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa). Abcam recommends using milk as the blocking agent - 3%. IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Target
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Function
Transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Contains 1 KRAB domain. -
Post-translational
modificationsUbiquitinated by PARK2. 'Lys-48'-linked polyubiquitination by PARK2 leads to degradation by the proteasome and may play a key role in regulation of neuron death. -
Cellular localization
Cytoplasm. Nucleus. Mainly localizes to the cytoplasm; probably translocates to the nucleus to repress selected genes. - Information by UniProt
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Database links
- Entrez Gene: 155061 Human
- Entrez Gene: 69228 Mouse
- Entrez Gene: 312303 Rat
- Omim: 613914 Human
- SwissProt: Q6NUN9 Human
- SwissProt: Q3U133 Mouse
- Unigene: 24643 Human
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Alternative names
- PARIS antibody
- Parkin-interacting substrate antibody
- Zinc finger protein 746 antibody
see all
Images
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Western blot - Anti-PARIS antibody (ab130867)All lanes : Anti-PARIS antibody (ab130867) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate with Human PARIS peptide (ab157686) at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 102 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Anti-PARIS antibody (ab130867) detects PARIS protein at ~100kDa, consistent with published data (PMID:21376232). This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab130867 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARIS antibody (ab130867)IHC image of PARIS staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab130867, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (3)
ab130867 has been referenced in 3 publications.
- Bae JH et al. ZNF746/PARIS overexpression induces cellular senescence through FoxO1/p21 axis activation in myoblasts. Cell Death Dis 11:359 (2020). PubMed: 32398756
- Chen CCW et al. Role of Parkin and endurance training on mitochondrial turnover in skeletal muscle. Skelet Muscle 8:10 (2018). PubMed: 29549884
- Chen CCW et al. Parkin is required for exercise-induced mitophagy in muscle: impact of aging. Am J Physiol Endocrinol Metab 315:E404-E415 (2018). PubMed: 29812989
Images
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Western blot - Anti-PARIS antibody (ab130867)All lanes : Anti-PARIS antibody (ab130867) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate with Human PARIS peptide (ab157686) at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 102 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Anti-PARIS antibody (ab130867) detects PARIS protein at ~100kDa, consistent with published data (PMID:21376232). This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab130867 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARIS antibody (ab130867)IHC image of PARIS staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab130867, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
