Anti-HSD11B1 antibody (ab39364)
Key features and details
- Rabbit polyclonal to HSD11B1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSD11B1 antibody
See all HSD11B1 primary antibodies -
Description
Rabbit polyclonal to HSD11B1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide:
CLELGAASAHYIAGT
, corresponding to amino acids 78/92 of Human HSD11B1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab39364 is an affinity purified IgG -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HSD11B1 antibody (ab39364) This image is courtesy of an Abreview submitted by Rob Cordery-Cotter. Experimental work performed by Megan A. Walton.
Western blot analysis of Sheep liver tissue lysate (5µg/lane) labeling HSD11B1 with ab39364 at 1/500 (16 hours at 4ºC). An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Anti-HSD11B1 antibody (ab39364) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Exposure time: 16 minutes
HSD11B1 contains a number of potential glycosylation sites (SwissProt) which may explain the higher migration pattern observed.