Anti-HSD11B1 antibody [EP9406(2)] (ab169785)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP9406(2)] to HSD11B1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-HSD11B1 antibody [EP9406(2)]
See all HSD11B1 primary antibodies -
Description
Rabbit monoclonal [EP9406(2)] to HSD11B1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide, corresponding to residues in Human HSD11B1 (Uniprot ID: P28845)
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Positive control
- Recombinant Human HSD11B1 protein (ab114301) can be used as a positive control in WB. Human fetal liver lysate. Raji lysate. Human kidney and liver tissues.
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General notes
Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP9406(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-HSD11B1 antibody [EP9406(2)] (ab169785) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Raji lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling HSD11B1 using ab169785 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling HSD11B1 using ab169785 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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