Anti-HSD11B1 antibody (ab84273)
Key features and details
- Rabbit polyclonal to HSD11B1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSD11B1 antibody
See all HSD11B1 primary antibodies -
Description
Rabbit polyclonal to HSD11B1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human HSD11B1 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab101308) -
Positive control
- Recombinant Human HSD11B1 protein (ab114301) can be used as a positive control in WB. This antibody gave a positive signal in Human liver tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).Target
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Function
Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol. -
Tissue specificity
Widely expressed. Highest expression in liver. -
Involvement in disease
Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 3290 Human
- Omim: 600713 Human
- SwissProt: P28845 Human
- Unigene: 195040 Human
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Alternative names
- 11 beta HSD 1 antibody
- 11 beta HSD1 antibody
- 11 beta hydroxysteroid dehydrogenase 1 antibody
see all
Images
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Western blot - Anti-HSD11B1 antibody (ab84273)Anti-HSD11B1 antibody (ab84273) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
HSD11B1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab84273 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-HSD11B1 antibody (ab84273)Anti-HSD11B1 antibody (ab84273) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
HSD11B1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
