Anti-Mitofilin antibody (ab229653)
Key features and details
- Rabbit polyclonal to Mitofilin
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Mitofilin antibody
See all Mitofilin primary antibodies -
Description
Rabbit polyclonal to Mitofilin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Mitofilin aa 76-215.
Database link: Q16891 -
Positive control
- WB: HeLa, HepG2 and MCF7 whole cell lysates. IHC-P: Human glioma cancer and kidney tissues. ICC/IF: HepG2 cells. IP: HepG2 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Mitofilin antibody (ab229653)All lanes : Anti-Mitofilin antibody (ab229653) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody (ab229653)Paraffin-embedded human glioma cancer tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody (ab229653)Paraffin-embedded human kidney tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody (ab229653)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mitofilin (green) using ab229653 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
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Immunoprecipitation - Anti-Mitofilin antibody (ab229653)Mitofilin was immunoprecipitated from 500 µg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with 8 µg ab229653.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in HepG2 whole cell lysate.
Lane 2: ab229653 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 10 µg.
For western blotting, an HRP-conjugated Protein G antibody was used as the Secondary antibody at 1/2000 dilution.
