Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
![Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)](https://www.abcam.com/ps/products/110/ab110329/Images/ab110329-123639-anti-mitofilin-antibody-2e4ad5-mitochondrial-marker-western-blot.jpg "Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)")
Key features and details
- Mouse monoclonal [2E4AD5] to Mitofilin - Mitochondrial Marker
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker
See all Mitofilin primary antibodies -
Description
Mouse monoclonal [2E4AD5] to Mitofilin - Mitochondrial Marker -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Human fibroblasts; Human cerebellum tissue; Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; HL60 cells.
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General notes
Western blot advice:
Hydrophobic intrinsic membrane proteins such as core mtDNA-encoded proteins tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.
Product was previously marketed under the MitoSciences sub-brand.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab110329 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
2E4AD5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329) at 2 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Lane 5 : HepG2 cell lysate at 20 µg
Predicted band size: 84 kDa
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs. -
![Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)](https://www.abcam.com/ps/products/110/ab110329/Images/ab110329-123641-anti-mitofilin-antibody-2e4ad5-mitochondrial-marker-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)Immunocytochemistry analysis using ab110329 at 5µg/ml staining Mitofilin in Cultured Human fibroblasts. (Fixed and permeabilized) followed by a fluorescent goat-anti-mouse IgG. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)](https://www.abcam.com/ps/products/110/ab110329/Images/ab110329-123640-anti-mitofilin-antibody-2e4ad5-mitochondrial-marker-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)Immunohistological analysis using ab110329 at 1/100 dilution staining Mitofilin in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded). -
![Flow Cytometry - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)](https://www.abcam.com/ps/products/110/ab110329/Images/ab110329-123638-anti-mitofilin-antibody-2e4ad5-mitochondrial-marker-flow-cytometry.gif)
Flow Cytometry - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)Flow cytometric analysis using ab110329 at 1µg/ml staining Mitofilin in HL60 cells (blue). Isotype control antibody (red). -
![Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)](https://www.abcam.com/ps/products/110/ab110329/Images/ab110329-295100-anti-mitofilin-antibody-2e4ad5-mitochondrial-marker-western-blot.jpg)
Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329) Image from Guarani V et al., Elife. 2016;5:e17163. Fig 5(A).; doi: 10.7554/eLife.17163. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
Lane 1 : Control neonatal mitochondria from skin fibroblasts
Lane 2 : Patient 2 (male) mitochondria from skin fibroblasts
Lane 3 : Patient 1 (female) mitochondria from skin fibroblasts
Predicted band size: 84 kDaClinical findings for patients 1 & 2 suggest human neonatal onset mitochondrial encephalopathy with recurrent bouts of liver disease.
