Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RECQ1 antibody [EPR8049(2)] (ab172607)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8049(2)] to RECQ1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-RECQ1 antibody [EPR8049(2)]
See all RECQ1 primary antibodies
Description

Rabbit monoclonal [EPR8049(2)] to RECQ1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RECQ1 aa 600 to the C-terminus. The exact sequence is proprietary.
Database link: P46063
Positive control
- HeLa, Raji, Ramos and 293T cell lysates; Human heart and thyroid gland carcinoma tissues; 293T and Ramos cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8049(2)
Isotype

IgG
Research areas

Western blot - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)

All lanes : Anti-RECQ1 antibody [EPR8049(2)] (ab172607) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 72 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling RECQ1 with ab172607 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)

Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling RECQ1 with ab172607 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)

Immunofluorescent analysis of 293T cells labeling RECQ1 with ab172607 at 1/250 dilution.
Flow Cytometry - Anti-RECQ1 antibody [EPR8049(2)] (ab172607)

Flow cytometric analysis of permeabilized Ramos cells labeling RECQ1 with ab172607 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).
Anti-RECQ1 antibody [EPR8049(2)] (ab172607)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com