Anti-APAF1 antibody [EPR21112-102] - BSA and Azide free (ab254248)
![Anti-APAF1 antibody [EPR21112-102] - BSA and Azide free (ab254248)](https://www.abcam.com/ps/products/254/ab254248/Images/ab254248-344420-AntiAPAF1-antibody-EPR21112102-immunoprecipitation-hek293t-human.jpg "Anti-APAF1 antibody [EPR21112-102] - BSA and Azide free (ab254248)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21112-102] to APAF1 - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-APAF1 antibody [EPR21112-102] - BSA and Azide free
See all APAF1 primary antibodies -
Description
Rabbit monoclonal [EPR21112-102] to APAF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: ab234436 IP in HEK-293T whole cell lysate. WB: Wild-type HAP1 whole cell lysate. HEK-293T, HeLa and A20 whole cell lysate.
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General notes
Ab254248 is the carrier-free version of ab234436. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab254248 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21112-102 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-APAF1 antibody [EPR21112-102] - BSA and Azide free (ab254248)
APAF1 was immunoprecipitated from 0.35 mg HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab234436 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234436 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: HEK-293T whole cell lysate 10 µg (Input).
Lane 2: ab234436 IP in HEK-293T whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234436 in HEK-293T whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234436).
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![Western blot - Anti-APAF1 antibody [EPR21112-102] - BSA and Azide free (ab254248)](https://www.abcam.com/ps/products/254/ab254248/Images/ab254248-344461-anti-apaf1-erp21112102-antibody-western-blot-knockout.jpg)
Western blot - Anti-APAF1 antibody [EPR21112-102] - BSA and Azide free (ab254248)All lanes : Anti-APAF1 antibody [EPR21112-102] (ab234436) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : APAF1 knockout HAP1 whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate
Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 141 kDaAb234436 was shown to specifically react with APAF1 in wild-type HAP1 cells as signal was lost in APAF1 knockout cells. Wild-type and APAF1 knockout samples were subjected to SDS-PAGE. ab234436 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.
Lanes 1,2 and 5 were developed using a higher sensitivity ECL substrate.
Exposure times: Lanes 1-2: 136 secs; Lanes 3-4: 3 mins; Lane 5: 136 secs.
Blocking/dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234436).
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![Anti-APAF1 antibody [EPR21112-102] - BSA and Azide free (ab254248)](https://www.abcam.com/ps/products/254/ab254248/Images/ab254248-3-benefits-of-recombinant-antibodies.png)
Anti-APAF1 antibody [EPR21112-102] - BSA and Azide free (ab254248)
