Anti-NDUFAF1 antibody [EPR2795] (ab92454)
![Anti-NDUFAF1 antibody [EPR2795] (ab92454)](https://www.abcam.com/ps/products/92/ab92454/Images/NDUFAF1-Primary-antibodies-ab92454-3.jpg "Anti-NDUFAF1 antibody [EPR2795] (ab92454)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2795] to NDUFAF1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-NDUFAF1 antibody [EPR2795]
See all NDUFAF1 primary antibodies -
Description
Rabbit monoclonal [EPR2795] to NDUFAF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human NDUFAF1 aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, 293T, A549 and HepG2 cell lysates. Human liver tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2795 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NDUFAF1 antibody [EPR2795] (ab92454)All lanes : Anti-NDUFAF1 antibody [EPR2795] (ab92454) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF1 antibody [EPR2795] (ab92454)](https://www.abcam.com/ps/products/92/ab92454/Images/NDUFAF1-Primary-antibodies-ab92454-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF1 antibody [EPR2795] (ab92454)Paraffin embedded Human liver tissue labelled with ab92454 at 1/100 dilution.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF1 antibody [EPR2795] (ab92454)](https://www.abcam.com/ps/products/92/ab92454/Images/NDUFAF1-Primary-antibodies-ab92454-5.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF1 antibody [EPR2795] (ab92454)Immunofluorescence analysis of HeLa cells labelled with ab92454 at 1/500 dilution. -
![Anti-NDUFAF1 antibody [EPR2795] (ab92454)](https://www.abcam.com/ps/products/92/ab92454/Images/ab92454-4-benefits-of-recombinant-antibodies.png)
Anti-NDUFAF1 antibody [EPR2795] (ab92454)
