Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8749] to Mitofilin - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Mitofilin antibody [EPR8749] - BSA and Azide free
    See all Mitofilin primary antibodies

  • Description

    Rabbit monoclonal [EPR8749] to Mitofilin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal heart and Human fetal muscle tissue lysates, HeLa, A673, and Saos 2 cell lysates; Human colon and Human testis tissues; HeLa cells

  • General notes

    ab245764 is the carrier-free version of ab137057 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab245764 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)
    Western blot - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)
    All lanes : Anti-Mitofilin antibody [EPR8749] (ab137057) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : IMMT knockout HAP1 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : Human Heart whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 84 kDa
    Observed band size: 84 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137057).

    Lanes 1 - 4: Merged signal (red and green). Green - ab137057 observed at 84 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab137057 was shown to specifically react with IMMT in wild-type HAP1 cells as signal was lost in IMMT knockout cells. Wild-type and IMMT knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab137057 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular cancer tissue sections labeling Mitofilin with purified ab137057 at 1/250 dilution (0.508 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137057).

  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)
    Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Mitofilin with purified ab137057 at 1/100 dilution (1.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137057).

  • Flow Cytometry - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)
    Flow Cytometry - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Mitofilin with purified ab137057 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137057).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling Mitofilin with purified ab137057 at 1/250 dilution (0.508 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137057).

  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)
    Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunofluorescent staining of HeLa cells labelling Mitofilin with ab137057 (unpurified) at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab137057) (unpurified) .

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunohistochemical analysis of paraffin embedded Human testis tissue labelling Mitofilin with ab137057 (unpurified) at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab137057) (unpurified).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Mitofilin with ab137057 (unpurified) at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab137057) (unpurified).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)
    Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

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