Anti-APAF1 antibody [EPR21112-102] (ab234436)
![Anti-APAF1 antibody [EPR21112-102] (ab234436)](https://www.abcam.com/ps/products/234/ab234436/Images/ab234436-344416-AntiAPAF1-antibody-EPR21112102-western-blot-fetal-heart-fetal-kidney-human.jpg "Anti-APAF1 antibody [EPR21112-102] (ab234436)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21112-102] to APAF1
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-APAF1 antibody [EPR21112-102]
See all APAF1 primary antibodies -
Description
Rabbit monoclonal [EPR21112-102] to APAF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB RatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal heart amd fetal kidney tissue lysate. Rat spleen and liver tissue lysate. Wild-type HAP1 whole cell lysate. HEK-293T, HeLa and A20 whole cell lysate. IP: ab234436 IP in HEK-293T whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21112-102 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-APAF1 antibody [EPR21112-102] (ab234436)All lanes : Anti-APAF1 antibody [EPR21112-102] (ab234436) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 141 kDa
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-APAF1 antibody [EPR21112-102] (ab234436)](https://www.abcam.com/ps/products/234/ab234436/Images/ab234436-344414-AntiAPAF1-antibody-EPR21112102-western-blot-spleen-liver-rat.jpg)
Western blot - Anti-APAF1 antibody [EPR21112-102] (ab234436)All lanes : Anti-APAF1 antibody [EPR21112-102] (ab234436) at 1/1000 dilution
Lane 1 : Rat spleen tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 141 kDaLanes 2 was developed using a higher sensitivity ECL substrate.
Exposure times: Lane 1: 169 secs; Lane 2: 3 mins.
Blocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-APAF1 antibody [EPR21112-102] (ab234436)](https://www.abcam.com/ps/products/234/ab234436/Images/ab234436-344413-AntiAPAF1-antibody-EPR21112102-western-blot-hap1-knockout-hek293t-hela-a20-human-mouse.jpg)
Western blot - Anti-APAF1 antibody [EPR21112-102] (ab234436)All lanes : Anti-APAF1 antibody [EPR21112-102] (ab234436) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : APAF1 knockout HAP1 whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate
Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 141 kDaAb234436 was shown to specifically react with APAF1 in wild-type HAP1 cells as signal was lost in APAF1 knockout cells. Wild-type and APAF1 knockout samples were subjected to SDS-PAGE. ab234436 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.
Lanes 1,2 and 5 were developed using a higher sensitivity ECL substrate.
Exposure times: Lanes 1-2: 136 secs; Lanes 3-4: 3 mins; Lane 5: 136 secs.
Blocking/dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-APAF1 antibody [EPR21112-102] (ab234436)](https://www.abcam.com/ps/products/234/ab234436/Images/ab234436-344412-AntiAPAF1-antibody-EPR21112102-immunoprecipitation-hek293t-human.jpg)
Immunoprecipitation - Anti-APAF1 antibody [EPR21112-102] (ab234436)APAF1 was immunoprecipitated from 0.35 mg HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab234436 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234436 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: HEK-293T whole cell lysate 10 µg (Input).
Lane 2: ab234436 IP in HEK-293T whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234436 in HEK-293T whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds. -
![Anti-APAF1 antibody [EPR21112-102] (ab234436)](https://www.abcam.com/ps/products/234/ab234436/Images/ab234436-5-benefits-of-recombinant-antibodies.png)
Anti-APAF1 antibody [EPR21112-102] (ab234436)