Complex I Immunocapture Kit (ab109711)
Key features and details
- Assay type: Quantitative
Overview
-
Product name
Complex I Immunocapture Kit
See all Complex I kits -
Assay type
Quantitative -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Product overview
Optimized for isolation of Complex I from small amounts of tissue for activity assays and other analyses. Based on a highly specific antibody.
250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 µg, ~120 µg or ~180 µg respectively of Complex I from heart mitochondria.
ab109711 allows for isolation of Complex I from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains NADH-ferricyanide, NADH-CoQ1 and NADH hexaaminoruthenium reductase activities after isolation, and with added lipids it also shows significant rotenone sensitivity. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Uses for ab109711 include but are not limited to examining alterations of Complex I subunits in inherited mitochondrial diseases, Parkinson's disease, Alzheimer's disease, ALS, schizophrenia, and aging.
Note: The immunocapture protocol for this kit requires Abcam detergent (lauryl maltoside, ab109857).
Product supplied as a 6.25% slurry. For the 250 µg, this consists of 250 µg antibody conjugated to 25 µL beads in a volume of 250 µL.
-
Notes
Related products
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 250 µg 500 µg Complex 1 monoclonal antibody coupled agarose beads 1 x 250µg 2 x 250µg -
Research areas
-
Alternative names
- NADH dehydrogenase
Images
-
Immunoprecipitation - Complex I Immunocapture Kit (ab109711)
Complex I was immunopurified from mitochondria isolated from human heart (HHM), cow/bovine heart (BHM), mouse heart (MHM) and mouse brain (MBM). The lanes were stained with Coomassie Brilliant Blue R. Bands were excised from the gel and proteolytically digested for mass spectrometry analysis.”
