Antibodies, Proteins, Kits and Reagents for Life Science

247 929 ₸ Product size

100 µl 247 929 ₸ 10 µl 100 512 ₸

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Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2478] to PDGFR alpha (phospho Y720)
Suitable for: WB, Flow Cyt
Reacts with: Mouse

Product name

Anti-PDGFR alpha (phospho Y720) antibody [EP2478]
See all PDGFR alpha primary antibodies
Description

Rabbit monoclonal [EP2478] to PDGFR alpha (phospho Y720)
Host species

Rabbit
Specificity

ab134068 only detects PDGFR alpha phosphorylated at Tyrosine 720.
Tested Applications & Species

Application Species
Flow Cyt
Mouse

WB
Mouse

See all applications and species data
Immunogen

corresponding to Human PDGFR alpha.
Positive control
- NIH 3T3 cell lysates, treated with Pervanadate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP2478
Isotype

IgG
Research areas

Western blot - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068)

All lanes : Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068) at 1/1000 dilution

Lane 1 : NIH 3T3 cell lysate, untreated
Lane 2 : NIH 3T3 cell lysate, treated with Pervanadate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 122 kDa
Observed band size: 190 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068)

Flow cytometry analysis of NIH/3T3 cells treated with 1mM Pervanadate for 30mins cells labeling PDGFR alpha (phospho Y720) with unpurified ab134068 at 1/200 dilution (0.0445µg) (red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730 /Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Flow Cytometry - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068)

Flow Cytometry analysis of permeabilized NIH/3T3 cells labelling PDGFR alpha (phospho Y720) with ab134068. Green - untreated, Red - pervanadate-treated, Blue - preincubated with phospho-PDGF Receptor alpha (phospho Y720) peptide, Orange - preincubated with non-phospho-PDGR Receptor alpha peptide.
Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068)

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