Anti-ErbB2 / HER2 antibody [4D5-8 (trastuzumab)] (ab245702)
![Anti-ErbB2 / HER2 antibody [4D5-8 (trastuzumab)] (ab245702)](https://www.abcam.com/ps/products/245/ab245702/Images/ab245702-336984-anti-erbb-2-antibody-her-2-neu-4d5-8-trastuzumab-immunohistochemistry.jpg "Anti-ErbB2 / HER2 antibody [4D5-8 (trastuzumab)] (ab245702)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [4D5-8 (trastuzumab)] to ErbB2 / HER2
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ErbB2 / HER2 antibody [4D5-8 (trastuzumab)]
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit monoclonal [4D5-8 (trastuzumab)] to ErbB2 / HER2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Tissue, cells or virus corresponding to ErbB2/ HER2. A431 cells (human epidermoid carcinoma) (over) expressing EGFR.
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Positive control
- IHC-P: Human breast tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4D5-8 (trastuzumab) -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [4D5-8 (trastuzumab)] (ab245702)
Paraffin-embedded human breast tissue stained for ErbB2 / HER2 with ab245702 at 4 µg/ml, 30 mins in immunohistochemical analysis. Antigen retreival was acheived by microwaving in citrate buffer (pH 6), followed by blocking with protein block serum-free buffer. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counterstaining with hemotoxylin.
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![Anti-ErbB2 / HER2 antibody [4D5-8 (trastuzumab)] (ab245702)](https://www.abcam.com/ps/products/245/ab245702/Images/ab245702-2-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 antibody [4D5-8 (trastuzumab)] (ab245702)
