Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho T6, mono methyl K9)
- Suitable for: WB, ICC/IF, ChIP, Dot blot
- Reacts with: Mouse, Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (phospho T6, mono methyl K9) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho T6, mono methyl K9) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, ChIP, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Human, Caenorhabditis elegans -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (phospho T6, mono methyl K9).
Database link: Q71DI3 -
Positive control
- WB: NIH/3T3 Histone prep.; C. elegans embryo lysate; HeLa Histone prep. ICC/IF: HeLa cells. ChIP: Chromatin prepared from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145) at 1/1000 dilution + HeLa Histone preparation at 30 µg
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)Immunofluorescent analysis of 0.5% PFA-fixed HeLa cells labeling Histone H3 (phospho T6, mono methyl K9) using ab272145 at 1/50 dilution for 1 hour at room temperature (Green). FITC secondary antibody at 1/10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue).
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Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145) at 1/1000 dilution + NIH/3T3 Histone preparation at 30 µg
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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ChIP - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)ChIP analysis of chromatin from one million formaldehyde cross-linked HeLa cells using 2µg of ab272145 and 20µl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Western blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145) at 1/1000 dilution + C. elegans embryo lysate at 30 µg
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
Other band(s): None.
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Dot Blot - Anti-Histone H3 (phospho T6, mono methyl K9) antibody (ab272145)Dot Blot analysis of Histone H3 (phospho T6, mono methyl K9) using ab272145 at 1/1000 dilution.
Lane 1: Histone H3 1-13.
Lane 2: T6p.
Lane 3: T6pK9Me1.
Lane 4: T6pK9Me2.
Lane 5: T6pK9Me3.
Lane 6: Histone H3 2-17.
Lane 7: K9Me1.
Lane 8: K9Me2.
Lane 9: K9Me3.
Lane 10: K9Ac.
Load: 1, 10, and 100 picomoles of peptide.