Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab30559)
Key features and details
- Rabbit polyclonal to Connexin 43 / GJA1 (phospho S368)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Connexin 43 / GJA1 (phospho S368) antibody
See all Connexin 43 / GJA1 primary antibodies -
Description
Rabbit polyclonal to Connexin 43 / GJA1 (phospho S368) -
Host species
Rabbit -
Specificity
Specific for ~43k Connexin43 protein phosphorylated at Ser368. -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer368 of rat Connexin43.
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Positive control
- Rat hippocampal lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab30559)All lanes : Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab30559) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate (lambda phosphatase treated)
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
The phosphospecificity of this labeling is shown in the second lane which was incubated in lambda phosphatase (1200 units for 30 min) before being exposed to GJA1.
