Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (ab78408)
![Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (ab78408)](https://www.abcam.com/ps/products/78/ab78408/Images/ab78408-94213-anti-connexin-45-gja7-cx45-antibody-5b92-immunocytochemistry.jpg "Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (ab78408)")
Key features and details
- Mouse monoclonal [5B9.2] to Connexin 45/GJA7/Cx45
- Suitable for: Flow Cyt, ICC
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2]
See all Connexin 45/GJA7/Cx45 primary antibodies -
Description
Mouse monoclonal [5B9.2] to Connexin 45/GJA7/Cx45 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC Rat
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Immunogen
Synthetic peptide corresponding to Human Connexin 45/GJA7/Cx45 aa 354-367 conjugated to keyhole limpet haemocyanin.
Sequence:QAYSHQNNPHGPRE
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Epitope
Near C terminal, cytoplasmic. -
Positive control
- ICC/IF: Connexin 45/GJA7/Cx45 transfected rat C6 cells. Flow Cyt: HeLa cells.
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General notes
Previously labelled as Connexin 45 / GJA7.
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
5B9.2 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (ab78408)
ab78408 staining Connexin 45/GJA7/Cx45 transfected rat C6 cells by Immunocytochemistry.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (ab78408)](https://www.abcam.com/ps/products/78/ab78408/Images/ab78408-94212-anti-connexin-45-gja7-cx45-antibody-5b92-flow-cytometry.jpg)
Flow Cytometry - Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (ab78408)Overlay histogram showing HeLa cells stained with ab78408 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78408, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
