Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)
![Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)](https://www.abcam.com/ps/products/52/ab52958/Images/ab52958-65511-anti-mek4-phospho-s80-antibody-ep1075y-immunohistochemistry.gif "Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)")
Key features and details
- Rabbit monoclonal [EP1075Y] to MEK4/MKK4 (phospho S80)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y]
See all MEK4/MKK4 primary antibodies -
Description
Rabbit monoclonal [EP1075Y] to MEK4/MKK4 (phospho S80) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human MEK4/MKK4 (N terminal). The exact sequence is proprietary.
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Positive control
- 3T3 cell lysates (untreated or treated with PDGF) and human muscle tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as MEK4.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1075Y -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)
ab52958 at 1/50 dilution staining MKK4 in human muscles by Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)](https://www.abcam.com/ps/products/52/ab52958/Images/ab52958-65512-anti-mek4-phospho-s80-antibody-ep1075y-western-blot.gif)
Western blot - Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)All lanes : Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958) at 1/5000 dilution
Lane 1 : 3T3 cell lysate untreated
Lane 2 : 3T3 cell lysate treated with PDGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
