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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway

Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)

Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to MEK4/MKK4 (phospho S80)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MEK4/MKK4 (phospho S80) antibody
    See all MEK4/MKK4 primary antibodies
  • Description

    Rabbit polyclonal to MEK4/MKK4 (phospho S80)
  • Host species

    Rabbit
  • Specificity

    ab131353 detects endogenous levels of MEK4/MKK4 only when phosphorylated at Serine 80.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MEK4/MKK4 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: NP_003001.1

  • Positive control

    • Methanol fixed HeLa cells; HepG2 cell extracts, treated with EGF; Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as MEK4

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS (without Mg2+and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131353 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Images

  • Western blot - Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)
    Western blot - Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)
    All lanes : Anti-MEK4/MKK4 (phospho S80) antibody (ab131353) at 1/500 dilution

    Lane 1 : HepG2 cell extracts, untreated
    Lane 2 : HepG2 cell extracts, treated with EGF

    Predicted band size: 44 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)
    Immunocytochemistry/ Immunofluorescence - Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)

    Immunofluorescent analysis of methanol fixed HeLa cells labelling MEK4/MKK4 (phospho S80) with ab131353 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)

    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MEK4/MKK4 (phospho S80) with ab131353 at 1/50 dilution (left). The image on the right is treated with blocking peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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