Anti-MEK4/MKK4 (phospho S80) antibody (ab131353)
Key features and details
- Rabbit polyclonal to MEK4/MKK4 (phospho S80)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MEK4/MKK4 (phospho S80) antibody
See all MEK4/MKK4 primary antibodies -
Description
Rabbit polyclonal to MEK4/MKK4 (phospho S80) -
Host species
Rabbit -
Specificity
ab131353 detects endogenous levels of MEK4/MKK4 only when phosphorylated at Serine 80.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MEK4/MKK4 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: NP_003001.1 -
Positive control
- Methanol fixed HeLa cells; HepG2 cell extracts, treated with EGF; Human breast carcinoma tissue.
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General notes
This product was previously labelled as MEK4
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS (without Mg2+and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131353 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MEK4/MKK4 (phospho S80) antibody (ab131353) at 1/500 dilution
Lane 1 : HepG2 cell extracts, untreated
Lane 2 : HepG2 cell extracts, treated with EGF
Predicted band size: 44 kDa
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Immunofluorescent analysis of methanol fixed HeLa cells labelling MEK4/MKK4 (phospho S80) with ab131353 at 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MEK4/MKK4 (phospho S80) with ab131353 at 1/50 dilution (left). The image on the right is treated with blocking peptide.