Biotin Anti-IgA antibody [RM220] (ab222777)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [RM220] to IgA
- Suitable for: ELISA, WB
- Reacts with: Mouse
- Conjugation: Biotin
Overview
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Product name
Biotin Anti-IgA antibody [RM220]
See all IgA primary antibodies -
Description
Biotin Rabbit monoclonal [RM220] to IgA -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length protein corresponding to Mouse IgA.
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Positive control
- WB: Nonreduced mouse IgA.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM220 -
Isotype
IgG -
Research areas
Images
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All lanes : Biotin Anti-IgA antibody [RM220] (ab222777) at 0.5 µg/ml
Lane 1 : Mouse IgA (non-reduced)
Lane 2 : Mouse IgA (reduced)ab222777 reacts with nonreduced IgA.
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ELISA of mouse immunoglobulins shows ab222777 reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. The plate was coated with 50 ng/well of different immunoglobulins. ab222777 at 200 ng/ml, 50 ng/ml or 10 ng/ml was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
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A titer ELISA of mouse IgA. The plate was coated with different amount of mouse IgA. A serial dilution of ab222777 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
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